Technical Data
C8452-72
Cyclin A2, NT
Description:
Cyclin A is a member of the Cyclin A family that can bind and activate Cdc2 and Cdk2 and is involved in the control of mitosis and S phase (1). During S phase, cyclin A forms a complex with Cdk2, the pRB-related protein p107 and E2F (2). Additionally, deregulation of cyclin A expression might contribute to tumor formation (3). The cyclin A family consist of two members, cyclin A1 and cyclin A2, to which cyclin A2 promotes both G1/S and G2/M transition (4).

Applications:
Suitable for use in Western Blot, Immunoprecipitation, Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blotting: 1:500
Immunocytochemistry: 1:250-500
Immunoprecipitation: 1:60

Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
TypeIsotypeCloneGrade
MabIgG6k136Supernatant
SizeStorageShippingSourceHost
100ul-20°CBlue IceHumanRabbit
Concentration:
Immunogen:
A synthetic peptide corresponding to residues near the N-term of human Cyclin A2
Purity:
Supernatant
Form
Supplied as a liquid
Specificity:
Recognizes human Cyclin A2
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Pines, J. & Hunter, T. (1990) Nature ( London) 346, 760-762.
2. Devoto, S. H., Mudryj, M., Pines, J., Hunter, T. & Nevins, J. R. (1992) Cell 68, 167-176.
3. Wang, J., Zindy, F., Chenivesse, X., Lamas, E., Henglein, B. & Brechot, C. (1992) Oncogene 7, 1653-1656.
4. Pagano, M.; Pepperkok, R.; Verde, F.; Ansorge, W.; Draetta, G. EMBO J. 11: 961-971, 1992.