		Technical Data
C8453-01E	CCNB1, phosphorylated (Ser126) (G2/Mitotic-specific Cyclin-B1, CCNB)

Description:
The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites.

Applications:
Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:100-1:500
Immunohistochemistry: 1:50-1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Rabbit

Concentration:

~0.25mg/ml

Immunogen:

Synthetic phosphopeptide corresponding to amino acid residues surrounding Ser126 of human CCNB1 (KLH).

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in PBS, 0.09% sodium azide.

Specificity:

Recognizes human CCNB1 when phosophorylated at Ser126.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Yoshida, T., et al., Ann. Oncol. 15(2):252-256 (2004). 2. Chang, D.C., et al., J. Biol. Chem. 278(39):37865-37873 (2003). 3. Sanchez, V., et al., J. Virol. 77(24):13214-13224 (2003). 4. Li, J.Q., et al., Int. J. Oncol. 22(5):1101-1110 (2003). 5. Porter, L.A., et al., Blood 101(5):1928-1933 (2003).