		Technical Data
C8453-01G	CCNB1, phosphorylated Ser128 (G2/Mitotic-specific Cyclin-B1, CCNB)

Description:
Cyclin B1 is a regulatory protein involved in mitosis. It complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts of the cyclin B1 gene have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase.

Applications:
Suitable for use in ELISA and Dot Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:1,000
Dot Blot: 1:500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Rabbit

Concentration:

~0.25mg/ml

Immunogen:

Synthetic phosphopeptide corresponding to amino acid residues surrounding Ser128 of human Cyclin B1 (KLH).

Purity:

Purified by Protein A affinity chromatography.

Form

Supplied as a liquid in PBS, 0.09% sodium azide.

Specificity:

Recognizes human Cyclin B1 when phosphorylated at Ser128.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1.Yoshida, T., et al., Ann. Oncol. 15(2):252-256 (2004). 2.Chang, D.C., et al., J. Biol. Chem. 278(39):37865-37873 (2003). 3.Sanchez, V., et al., J. Virol. 77(24):13214-13224 (2003). 4.Li, J.Q., et al., Int. J. Oncol. 22(5):1101-1110 (2003). 5.Porter, L.A., et al., Blood 101(5):1928-1933 (2003).