		Technical Data
C8453-01J	CCNB1 (S126) (G2/Mitotic-specific Cyclin-B1, CCNB)

Description:
Cyclin B1 is a regulatory protein involved in mitosis. It complexes with p34(cdc2) to form the maturation-promoting factor (MPF).

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:50-1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Rabbit

Concentration:

~0.25mg/ml

Immunogen:

Synthetic peptide corresponding to amino acid residues surrounding S126 of human CCNB1 (KLH).

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in PBS, 0.09% sodium azide.

Specificity:

Recognizes human CCNB1. Species Crossreactivity: mouse.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1.Yoshida, T., et al., Ann. Oncol. 15(2):252-256 (2004). 2.Chang, D.C., et al., J. Biol. Chem. 278(39):37865-37873 (2003). 3.Sanchez, V., et al., J. Virol. 77(24):13214-13224 (2003). 4.Li, J.Q., et al., Int. J. Oncol. 22(5):1101-1110 (2003). 5.Porter, L.A., et al., Blood 101(5):1928-1933 (2003).