		Technical Data
C8453-04E	CCNB1 (Cyclin B1, G2/Mitotic-specific Cyclin-B1, CCNB)

Description:
The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1ug/ml
Immunohistochemistry (Formalin fixed paraffin embedded): 10ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG1	V63	Affinity Purified

Size		Storage	Shipping	Source	Host
50ug		-20°C	Blue Ice	Hamster	Mouse

Concentration:

As reported

Immunogen:

Purified hamster cyclin B1

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol.

Specificity:

Recognizes hamster CCNB1. Species sequence homology: Canine, guinea pig, human, mouse, porcine, rabbit, rat, sheep.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.