Technical Data
C8453-04E
CCNB1 (Cyclin B1, G2/Mitotic-specific Cyclin-B1, CCNB)
Description:
The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1ug/ml
Immunohistochemistry (Formalin fixed paraffin embedded): 10ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1V63Affinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceHamsterMouse
Concentration:
As reported
Immunogen:
Purified hamster cyclin B1
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol.
Specificity:
Recognizes hamster CCNB1. Species sequence homology: Canine, guinea pig, human, mouse, porcine, rabbit, rat, sheep.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.