		Technical Data
C8453-06	Cyclin B1

Description:
Applications:
Suitable for use in Immunoblot and Immunoprecipitation. Other applications not tested.

Recommended DilutionWarren
Immunoblot: 0.5-5ug/ml detecs cyclin B1 in RIPA lysates from human A431 carcinoma cells.
Immunoprecipitation: 2ug precipitated human cyclin B1 and cdc2 kinase from 500ug of A431 RIPA lysate.
Optimal dilutions to be determined by the researcher.

Positive Antigen Control: A0001-50
Add 2.5ul of 2-mercaptoethanol/100ul of lysate and boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane for minigels.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgG2b	0.T.183	Affinity Purified

Size		Storage	Shipping	Source	Host
200ug		4°C (-20°C Glycerol)	Blue Ice	Human	Mouse

Concentration:

~1mg/ml

Immunogen:

Recombinant human cyclin B1.

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as liquid in 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide.

Specificity:

Specific for human cyclin B1 (~ 58kD). Does not crossreact with cyclin A or cyclin D. Species crossreactivity: Human, weak species crossreactivity with mouse was observed..

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.