		Technical Data
C8453-07	Cyclin B1

Description:
The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product forms a complex with p34 (cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript that is
expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites.

Applications:
Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:2000-1:10,000
Western Blot: 1:500-1:1000
Immunoprecipitation: 1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Serum

Size		Storage	Shipping	Source	Host
100ul		-20°C	Blue Ice	Human	Rabbit

Concentration:

Not determined

Immunogen:

Full length GST fusion protein corresponding to human Cyclin B1.

Purity:

Purified by delipidation and de brination.

Form

Supplied as a liquid, 0.01% sodium azide. No stabilizing proteins added.

Specificity:

Recognizes human Cyclin B1 between 55-62kD. No reaction was observed against other related cyclins. Species Crossreactivity: rat and mouse.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.