Technical Data
C8453-07
Cyclin B1
Description:
The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product forms a complex with p34 (cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript that is
expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites.

Applications:
Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:2000-1:10,000
Western Blot: 1:500-1:1000
Immunoprecipitation: 1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Full length GST fusion protein corresponding to human Cyclin B1.
Purity:
Purified by delipidation and de brination.
Form
Supplied as a liquid, 0.01% sodium azide. No stabilizing proteins added.
Specificity:
Recognizes human Cyclin B1 between 55-62kD. No reaction was observed against other related cyclins. Species Crossreactivity: rat and mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.