		Technical Data
C8453-10X	Cyclin B1 (G2/Mitotic-specific Cyclin-B1, CCNB, Ccnb1, Ccn-2, Ccnb1-rs13, Cycb, Cycb1) (BSA & Azide Free)

Description:
Cyclin B1 belongs to the cyclin family, Cyclin AB subfamily, and predicted molecular weight 48kD. Cyclin B1 is expressed ubiquitously in all eukaryotes and the level of cyclin B1 was minimal in G, began to rise in S phase, and is primarily cytoplasmic. Cyclin B1 moves into the nucleus just at the start of mitosis and associates with condensed chromosomes and the mitotic spindle. The level of cyclin B1 peaked at the G/M transition. Cyclin B1 is essential for the control of the cell cycle at the G2/M transition.

Applications:
Suitable for use in Flow Cytometry, Western Blot and Immunohistochemistry. Not suitable for use in Immunoprecipatation. Other applications not tested.

Recommended Dilution:
Western Blot: 1-2ug/ml for 2 hrs at RT
Immunohistochemistry (Formalin/paraffin): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 minutes followed by cooling at RT for 20 minutes.
Optimal dilutions to be determined by the researcher.

Positive Control:
Jurkat cells. Tonsil or breast carcinoma

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgG1	V152	Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Hamster	Mouse

Concentration:

1mg/ml

Immunogen:

Hamster cyclin B1 protein.

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as a liquid in 10mM PBS, pH 7.4. Also available with BSA and azide. See C8453-10.

Specificity:

Recognizes hamster Cyclin B1 at ~62kDa. Species Crossreactivity: human, mouse and rat

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

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