Technical Data
C8453-15
Cyclin B1
Description:
In mammals, cyclin B associates with inactive p34-cdc2. Cyclin B-p34-cdc2 plays a critical role in G2 to M transition.

Applications:
Suitable for use in Flow Cytometry, Immunofluorescence, Immunoprecipitation and Western Blot. Other applications have not been tested.

Recommended Dilution:
Immunoprecipitation: 2ug antibody/mg protein lysate. (Use Protein G under native and denatured conditions).
Western Blot: 1-2ug/ml for 2 hours at RT.
Optimal dilutions to be determined by the researcher.

Positive Control:
MAD109 or LS174T cells

Source:
Ascites

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG10.N.238Affinity Purified
SizeStorageShippingSourceHost
500ul4°C (-20°C Glycerol)Blue IceHumanMouse
Concentration:
~0.2mg/ml
Immunogen:
Human cyclin B1 protein. MW:62kD Epitope: aa1-21. Cellular Localization: Cytoplasmic.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide at 1mg/ml. See C8453-15X.
Specificity:
Recognizes Cyclin B1 at 62kD. Species Reactivity: Human, mouse and hamster.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
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