Technical Data
C8453-20
Cyclin B1
Description:
In mammals, cyclin B associates with inactive p34cdc2 which facilitates phosphorylation of p34cdc2 at aa 14Thr and 15Tyr. This maintains the inactive state until the end of G2-phase. The inactive cyclin B-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates aa 14Thr and 15Tyr of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when cyclin B is degraded. This degradation process is ubiquitin-dependent and is necessary for the cell to exit mitosis. So, cyclin B-p34cdc2 plays a critical role in G2 to M transition. Ab-2 is excellent for biochemical applications ans co-precipitates cyclin B1 associated p34cdc2.

Positive Control: Hela or NIH-3T3 cells.

Applications and Suggested Dilutions:
Immunoprecipitation
(Co-precipitates cdk1/p34cdc2)

Cellular Localization: Cytoplasmic
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
500ul-20CBlue IceHumanRabbit
Concentration:
1mg/ml
Immunogen:
KLH peptide corresponding to aa 420-433 (Cys-SALVQDLAKAVAKV) from the C-terminal of human cyclin B1.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in 10mM PBS, pH 7.4, 15mM sodium azide, 0.2% BSA. Also supplied without BSA and sodium azide. See C8453-20X.
Specificity:
Cyclin B1 Ab-2
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.