Technical Data
C8453-31
Cyclin E
Description:
Cyclin E is a regulatory subunit of cdk-2 and controls G1/S transition during the mammalian cell cycle. Multiple isoforms of cyclin E are expressed only in tumors but not in the normal tissues. Qualitative and quantitative alterations in cyclin E protein have been implicated as indicators of worse prognosis in various cancers.

Applications:
Suitable for use in Immunohistochemistry, ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000-1:5000
ELISA: 1:50,000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
100ul4C (-20C Glycerol)Blue IceHumanRabbit
Concentration:
Not determined.
Immunogen:
Synthetic peptide corresponding to the C-terminal of human Cyclin E (KLH).
Purity:
Serum.
Form
Supplied as a lyophilized powder in PBS, pH 7.2, 0.1% sodium azide before the addition of glycerol to 40%. No stabilizing proteins added.
Specificity:
Recognizes human Cyclin E at 45kD. IEP Assay shows a single precipitin arc against source serum protein and IgG. No reaction was observed against other related cyclins.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.