		Technical Data
C8455-01J	CCNE1, phosphorylated (Thr395) (G1/S-specific Cyclin-E1, CCNE)

Description:
Cyclin E1 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. Cyclin E1 forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. Cyclin E1 accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of Cyclin E1 has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB.

Applications:
Suitable for use in ELISA, Western Blot, Dot Blot, and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:100-1:500
Immunohistochemistry: 1:50-1:100
Dot Blot: 1:500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Rabbit

Concentration:

~0.25mg/ml

Immunogen:

Synthetic phosphopeptide corresponding to amino acid residues surrounding Thr395 of human Cyclin E1 (KLH).

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in PBS, 0.09% sodium azide.

Specificity:

Recognizes human Cyclin E1 when phosphorylated at Thr395.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Ausserlechner, M.J., et al., Leukemia 19(6):1051-1057 (2005). 2. Wingate, H., et al., J. Biol. Chem. 280(15):15148-15157 (2005). 3. Honda, R., et al., EMBO J. 24(3):452-463 (2005). 4. Brzezinski, J., et al., Clin. Cancer Res. 11(3):1037-1043 (2005). 5. Hayami, R., et al., Cancer Res. 65(1):6-10 (2005).