Technical Data
C8455-01K
CCNE1, CT (G1/S-specific Cyclin-E1, CCNE)
Description:
Cyclin E1 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. Cyclin E1 forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. Cyclin E1 accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of Cyclin E1 has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB.

Applications:
Suitable for use in Immunohistochemistry, Flow Cytrometry and Western Blot. Other applications have not been tested.

Recommended Dilution:
Immunohistochemistry (formalin-fixed, parafin-embedded): 1:50-1:100
Flow Cytrometry: 1:10-1:50
Western Blot: 1:50-1:100
Optimal dilutions to be determined by researcher.

Positive Control:
A2058 cell line lysates, human testis tissue and NCI-H292 cells.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Synthetic peptide corresponding to the C-terminal region of human Cyclin E1 conjugated to KLH (P24864).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity:
Recognizes human Cyclin E1 at ~47kD.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Ausserlechner, M.J., et al., Leukemia 19(6):1051-1057 (2005). 2.Wingate, H., et al., J. Biol. Chem. 280(15):15148-15157 (2005). 3.Honda, R., et al., EMBO J. 24(3):452-463 (2005). 4.Brzezinski, J., et al., Clin. Cancer Res. 11(3):1037-1043 (2005). 5.Hayami, R., et al., Cancer Res. 65(1):6-10 (2005).