Technical Data
C8455-10X
Cyclin E (BSA & Azide Free)
|
Technical Data |
|
C8455-10X |
Cyclin E (BSA & Azide Free) |
|
|
Description: Cyclin E is a regulatory subunit of cdk-2 and controls G1/S transition during the mammalian cell cycle. Multiple isoforms of cyclin E are expressed only in tumors but not in the normal tissues. Qualitative and quantitative alterations in cyclin E protein have been implicated as indicators of worse prognosis in various cancers. Applications: Suitable for use in Flow Cytometry, Immunofluorescence, Western Blot and Immunohistochemistry. Not suitable for use in Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1-2ug/ml for 2 hours at RT. A doublet of 50kD and a single band of 42kD are observed. Immunohistochemistry (Formalin/paraffin): 2-4ug/ml for 30 minutes at RT. Staining of formalin/paraffin tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilutions to be determined by the researcher. Positive Control: SKBR3 or LS174T cells. Breast carcinomas or placenta. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
|
|||||||||||
|
||||||||||||
|
||||||||||||
|
||||||||||||