Technical Data
C8455-10X
Cyclin E (BSA & Azide Free)
Description:
Cyclin E is a regulatory subunit of cdk-2 and controls G1/S transition during the mammalian cell cycle. Multiple isoforms of cyclin E are expressed only in tumors but not in the normal tissues. Qualitative and quantitative alterations in cyclin E protein have been implicated as indicators of worse prognosis in various cancers.

Applications:
Suitable for use in Flow Cytometry, Immunofluorescence, Western Blot and Immunohistochemistry. Not suitable for use in Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Western Blot: 1-2ug/ml for 2 hours at RT. A doublet of 50kD and a single band of 42kD are observed.
Immunohistochemistry (Formalin/paraffin): 2-4ug/ml for 30 minutes at RT. Staining of formalin/paraffin tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes.
Optimal dilutions to be determined by the researcher.

Positive Control:
SKBR3 or LS174T cells. Breast carcinomas or placenta.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG10.N.244Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
1mg/ml
Immunogen:
Recombinant human cyclin E protein. Cellular Localization: Nuclear
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in 10mM PBS, pH 7.4. Also available with BSA and azide. See C8455-10.
Specificity:
Recognizes human Cyclin E. Does not react with mouse or rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Faha B, et. al. J Virology, 1993;67:2456-2465. 2. Keyomarsi K, et. al. Cancer Research, 1994; 54:380-385. 3. Gorospe M, et. al. Mol Cell Biol, 1996; 16:762-770. 4. Scuderi R, et. al. Blood, 1996; 87:3360-3367. 5. Gong J, et. al. Int J Oncology, 1993; 3:1037-1042 6. Gong J, et. al. Cancer Research, 1993; 53:5096-5099. 7. Gong J, et. al. Cell Proliferation, 1995; 28:1-32. 8. Gong J, et. al. Leukemia, 1995; 9:893-899. 9. Darzynkiewicz ZJ, et. al. Cytometry, 1996; 25:1-13.