Technical Data
C8471-62A
CDKN1B (CDKN1B, cyclin-dependent kinase inhibitor 1B (p27, Kip1), KIP1, CDKN4, P27KIP1, cyclin-dependent kinase inhibitor 1B, p27(KIP1), KIP1, p27)
Description:
This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state.

Positive Control: Human Breast lysate.

Applications:
Suitable for use in ELISA (peptide) and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA (peptide): 1:32000
Western Blot: 1-3ug/ml. ~ 26kD band observed in Human Breast and Prostate lysates (calculated MW of 22.1kD according to NP_004055.1).
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide, EQTPKKPGLRRRQT, representing the C Terminus of the human protein according to NP_004055.1.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in Tris-saline, pH7.2, 0.5% BSA.
Specificity:
Species Crossreactivity: Human. Other species not tested. Expected based upon sequence homology: Mouse, Rat, bovine, canine
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Toyoshima H, Hunter T p27, a novel inhibitor of G1 cyclin-Cdk protein kinase activity, is related to p21. Cell. 1994 Jul 15;78(1):67-74. PMID: 8033213