Technical Data
C8850
Cyclophilin A (Cyclosporin A Binding Protein, Peptidyl Prolyl Cis Trans Isomerase A, PPIASE)
Description:
Immunophilins are a family of soluble cytosolic receptors capable of binding to one of two major immunosuppressant agents - cyclosporin A (CsA) or FK506. Proteins that bind FK506 are termed FK506 Binding Proteins (FKBPs) and those that bind cyclosporin A are called cyclophilins (CyP). Both CyP:CsA and FKBP:FK506 complexes have been shown to inhibit calcineurin, a calcium and calmodulin dependent protein phosphatase which has been implicated as an important signaling enzyme in T-cell activation, providing a possible mechanism of immunosuppression by CsA and FK506. Within the cyclophilin family, there are several different proteins which show a high degree of homology including CyPA, CyPB and CyPC. CyPA, also termed CyP-18, is the most abundant and ubiquitous cyclophilin found in all vertebrate tissues and is present in T-cells.

Applications:
Suitable for use in Western Blot. Other applications have not been tested.

Recommended Dilution:
Western Blot: 1:1000-1:10,000 detects Cyclophilin A in RIPA lysates from nonstimulated A431 cells. A previous lot detected Cyclophilin A in RIPA lysates from rat lung cells. 20ug of A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with C8850 (1:10,000). Proteins were visualized using a Gt xRb IgG (HRP)* and a chemiluminescence detection system.
Optimal dilutions to be determined by the researcher.

*Recommended Secondary Antibodies:
I1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb
I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb
I1904-46Q: IgG, H&L (HRP) Pab Gt xRb

Included Positive Antigen Control:
A0001-50: Non stimulated A431 cell lysate

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
50ul-20CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Recombinant full length protein corresponding to human cyclophilin A.
Purity:
Serum
Form
Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, 30% glycerol.
Specificity:
Recognizes human cyclophilin A (18kD). Additional bands may be detected at higher concentrations of the antibody. Species Crossreactivity: rat, mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
US Biological application reference: 1. Yurchenko, Y. et al., (2005)J. Biol. Chem. 280:17013-17019. 2. Zhao, Y. et al., (2004) J. Physiology 558: 623-632. 3. Pushkarsky, T. et al.,(2005) J. Biol. Chem. 280:27866-27871. 1. Lee, S., et al., J. Biol. Chem. 276: 29,826-29,832 (2001). 2. Haendler, B., et al., EMBO J. 6: 947950 (1987). 3. Pfuegel, G., et al., Nature 361: 9194 (1993).