Technical Data
C8850-01E
Cyclophilin A (CYPA, Cyclosporin A-binding Protein, CYPH, HGNC:9253, MGC12404, MGC23397, MGC117158, Peptidyl-prolyl cis-trans Isomerase A, Peptidyl Prolyl Cis Trans Isomerase A, Peptidylprolyl Isomerase A (Cyclophilin-A), PPIA, PPIase A, Rotamase A, Rotam
Description:
This gene encodes a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. The encoded protein is a cyclosporin binding-protein and may play a role in cyclosporin A-mediated immunosuppression. The protein can also interact with several HIV proteins, including p55 gag, Vpr, and capsid protein, and has been shown to be necessary for the formation of infectious HIV virions. Multiple pseudogenes that map to different chromosomes have been reported.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:500-1:1000
ELISA: 1-3ng/ml
Optimal dilutions to be determined by the researcher.

Hybridoma:
Sp2/0 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2a,k10B2627Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Full-length recombinant protein corresponding to aa1-166 of human Cyclophilin A with GST tag. MW of GST tag alone is 26kD.
Purity:
Purified by ammonium sulfate precipitation.
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added (Azide free).
Specificity:
Recognizes human Cyclophilin A.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.