Technical Data
C8850-01G
Cyclophilin A (CYPA, Cyclosporin A-binding Protein, CYPH, HGNC:9253, MGC12404, MGC23397, MGC117158, Peptidyl-prolyl cis-trans Isomerase A, Peptidyl Prolyl Cis Trans Isomerase A, Peptidylprolyl Isomerase A (Cyclophilin-A), PPIA, PPIase A, Rotamase A, Rotam
Description:
PPIA is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein is a cyclosporin binding-protein and may play a role in cyclosporin A-mediated immunosuppression. The protein can also interact with several HIV proteins, including p55 gag, Vpr, and capsid protein, and has been shown to be necessary for the formation of infectious HIV virions.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 0.1-0.3ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
Human Lymph lyssate

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to C-ERFGSRNGKTSKK, from the internal region of the protein sequence according to NP_066953.1.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH7.3, 0.5% BSA.
Specificity:
Recognizes human Cyclophilin A. Species sequence homology: canine, mouse, rat, porcine, bovine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Berthoux L, Sebastian S, Sokolskaja E, Luban J. Cyclophilin A is required for TRIM5{alpha}-mediated resistance to HIV-1 in Old World monkey cells. Proc Natl Acad Sci USA. 2005 Oct 11;102(41):14849-53. Epub 2005 Oct 3. PMID: 16203999.