		Technical Data
C8850-01H	Cyclophilin A (Peptidyl-prolyl Cis-trans Isomerase A, PPIase A, Rotamase A, PPIA, Cyclosporin A-binding Protein, CYPA)

Description:
PPIA is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein is a cyclosporin binding-protein and may play a role in cyclosporin A-mediated immunosuppression. The protein can also interact with several HIV proteins, including p55 gag, Vpr, and capsid protein, and has been shown to be necessary for the formation of infectious HIV virions.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:8000
Western Blot: 0.1-0.3ug/ml, observed in Human Lymph Node lysates on ~18kD bands
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab			Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Goat

Concentration:

~0.5mg/ml

Immunogen:

Synthetic peptide corresponding to C-ERFGSRNGKTSKK, from human PPIA, at the internal region of the protein (NP_066953.1).

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.

Specificity:

Recognizes human PPIA. Species sequence homology: Bovine, canine, mouse, porcine and rat.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Berthoux L, Sebastian S, Sokolskaja E, Luban J. Cyclophilin A is required for TRIM5{alpha}-mediated resistance to HIV-1 in Old World monkey cells. Proc Natl Acad Sci USA 2005 Oct 11;102(41):14849-53. Epub 2005 Oct 3.