Technical Data
Cyclophilin A (Cyclosporin A Binding Protein, Peptidyl Prolyl Cis Trans Isomerase A, PPIASE)
Immunophilins are a family of soluble cytosolic receptors capable of binding to one of two major immunosuppressant agents-cyclosporin A (CsA) or FK506. Proteins that bind FK506 are termed FK506 Binding Proteins (FKBPs) and those that bind cyclosporin A are called cyclophilins (CyP).
Both CyP:CsA and FKBP:FK506 complexes have been shown to inhibit calcineurin, a calcium and calmodulin dependent protein phosphatase which has been implicated as an important signaling enzyme in T-cell activation, providing a possible mechanism of immunosuppression by CsA and FK506. Immunophilins function as peptidyl prolyl cis-trans-isomerases (PPIase) whose activity is inhibited by their respective immunosuppressant compounds. As PPIase's, immunophilins accelerate folding of some proteins both in vivo and in vitro by catalyzing slow steps in the initial folding and rearrangement of proline containing proteins.
Within the cyclophilin family, there are several different proteins which show a high degree of homology including CyPA, CyPB and CyPC. CyPA, also termed CyP-18, is the most abundant and ubiquitous cyclophilin found in all vertebrate tissues and is present in T-cells. Cyclophilin A is a cyclosporin A binding protein that catalyzes the isomerization of proline imidic peptide bonds and is also known as peptidyl-prolyl cis-trans isomerase A (PPIASE). As a cytoplasmic enzyme, it accelerates the folding of proteins.

Suitable for use in Western Blot. Other applications have not been tested.

Recommended Dilution:
Western Blot: 1:150
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
100ul4C (-20C Glycerol)Blue IceHumanRabbit
Not determined
Recombinant human Cyclophilin A expressed in E. coli.
Purified by ammonium sulfate precipitation.
Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide, before the addition of glycerol to 40%.
Recognizes recombinant human Cyclophilin A at ~18kD, as well as CyPa protein from CHO cells. Does not detect endogenous levels. Species Crossreactivity: Hamster.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Haendler, B., et al., EMBO J. 6: 947-950 (1987). 2. Pfuegel, G., et al., Nature 361: 91-94 (1993). 3. J. Biol. Chem. 272(10): 6677-6684 (1997). 4. J. Biol. Chem. 269(29): 18,877-18,880 (1994). 5. J. Virol. 79(1): 176-183 (2005). 6. Toxicological Sciences 79: 266-277 (2004). 7. J. Viol. 77: 9052-9068 (2003). 8. Ren Bartz, R., et al., Mol. Biol. Cell 18: 3015-3025 (2007). 9. Lou, J., et al., Cancer Epidemiol. Biomarkers Prev. 15: 1598-1606 (2006).