		Technical Data
C8855	Cyclophilin B (PPIB, Peptidylprolyl isomerase B (cyclophilin B), Cyclophilin B, CYPB, CYP-S1, MGC14109, MGC2224, Peptidyl-prolyl cis-trans isomerase B, PPIase, Rotamase, S-cyclophilin, SCYLP)

Description:
Cyclophilin B (CyPB) is a cyclosporin A-binding protein and a peptidyl prolylisomerase (PPI) that is a member of the immunophilin family of soluble cytosolic receptors and mainly localized within the endoplasmic reticulum vesicles.1 Cyclophilin B is secreted in response to inflammatory stimuli and CD147 serves as one its receptors.2 The CyPB-cyclosporin A complex has been shown to inhibit calcineurin, a calcium and calmodulin dependent protein phosphatase which has been implicated as an important signaling enzyme in T-cell activation. A recent study has shown that CyPB is important for facilitating the nuclear retrotransport and action of the somatolactogenic hormone family, such as prolactin and growth hormone.3 CyPB is necessary for the prolactin-induced proliferation, cell growth, and the nuclear retrotransport of prolactin. The prolactin/ CyPB complex acts as a transcriptional inducer by interacting directly with Stat5, which can ultimately enhance Stat5 DNA-binding activity and prolactin-induced, Stat5- mediated gene expression.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilutions:
ELISA: 0.1-1ug/ml
Western Blot: 1-3ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG1,k	3G118	Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Mouse

Concentration:

~0.5mg/ml

Immunogen:

Recombinant protein encompassing the C-terminal region of human Cyclophilin B.

Purity:

Purified by Protein A affinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.

Specificity:

Recognizes the ~20kD human Cyclophilin B protein. Reactivity was con rmed with a Cyclophilin B-transfected 293 cell line as well as T-47D and MCF-7 breast carcinoma cell lines.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Allain F, et al. J Biol Chem 269(24) : 16537-40, 1994. 2. Yurchenko V, et al. Biochem Biophys Res Commun 288(4) : 786-8, 2001. 3. Rycyzyn MA and Clevenger CV. Ann NY Acad Sci 917: 514-521, 2000. 4. Rycyzyn MA and Clevenger CV. PNAS. 99(10): 6790-6795, 2002.