Technical Data
C8855-86
Cyclophilin H (Peptidyl Prolyl Cis Trans Isomerase H, PPIASE, PPIH)
Description:
This protein is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein is a specific component of the complex that includes pre-mRNA processing factors PRPF3, PRPF4, and PRPF18, as well as U4/U5/U6 tri-snRNP. This protein has been shown to possess PPIase activity and may act as a protein chaperone that mediates the interactions between different proteins inside the spliceosome.

Applications:
Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: > 1:250
Immunohistochemistry, Immunocytochemistry: > 1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgYAffinity Purified
SizeStorageShippingSourceHost
50ug4°C (-20°C Glycerol)Blue IceHumanChicken
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to aa1-177 of human Cyclophilin H. Protein GI Number: 545154 Protein Accession Number: NP_006338.1
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
Specificity:
Recognizes human Cyclophilin H. Species Crossreactivity: Human and mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Reidt, U., Wahl, M.C., Fasshauer, D., Horowitz, D.S., Luhrmann, R., Ficner, R., Crystal structure of a complex between human splicesomal cyclophilin H and a U4/U6 snRNP-60K oeotude, J. Mol. Bio. 331(1): 45-56 (2003).