		Technical Data
C8900-01	Cyclosporin A (Antibiotic S 7481F1, Ciclosporin A, Ramihyphin A, CsA)

Description:
Cyclic hydrophobic oligopeptide isolated from the filamentous fungi, Tolypocladium inflatum, with potent immunosuppressant properties. Induces apoptosis in rat thymocytes and in murine B cell lymphoma cells, but prevents apoptosis in BLB cell lines induced by anti-IgM and ionomycin. The complex of cyclosporin A with cyclophilin inhibits T-cell receptor signal transduction pathway via the inhibition of calcineurin (protein phosphatase 2B) with nanomolar affinity (Ki270nM). Cyclosporin A inhibits interleukin-1a-, lipopolysaccharide- and TNF-a-nitric oxide synthesis induction.

Applications:
Suitable for use in ELISA. Other applications have not been tested.

Recommended Dilutions:
Optimal dilutions to be determined by the researcher.

Kd: 1x10e-8 L/mol

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG1	1.BB.884	Affinity Purified

Size		Storage	Shipping	Source	Host
1mg		-20°C	Blue Ice		Mouse

Concentration:

~2mg/ml

Immunogen:

Cyclosporin C (BSA coupled). Cyclosporin C is a derivative of Cyclosporin A.

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.

Specificity:

Recognizes Cyclosporin A in buffer solutions and whole blood extracts.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.