Technical Data
C8929-03B
CYP17A1, ID (Steroid 17-alpha-hydroxylase/17,20 Lyase, Steroid 17-alpha-Monooxygenase, Cytochrome P450-C17, Cytochrome P450c17, CYPXVII, Cytochrome P450 17A1, Cytochrome P450-C17, Cytochrome P450c17, CYP17, S17AH)
Description:
CYP17A1 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum. It has both 17alpha-hydroxylase and 17,20-lyase activities and is a key enzyme in the steroidogenic pathway that produces progestins, mineralocorticoids, glucocorticoids, androgens, and estrogens. Mutations in CYP17A1 gene are associated with isolated steroid-17 alpha-hydroxylase deficiency, 17-alpha-hydroxylase/17,20-lyase deficiency, pseudohermaphroditism, and adrenal hyperplasia.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:50-1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGPurified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Synthetic peptide selected from the Center region of human CYP17A1 (KLH).
Purity:
Purified by ammonium sulfate precipitation.
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity:
Recognizes human CYP17A1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Yuan,X., Cancer Epidemiol. Biomarkers Prev. 17(12), 3621-3627 (2008). 2.Nelson,D.R., Pharmacogenetics 14(1), 1-18 (2004). 3.Imai,T., Hum. Genet. 89(1), 95-96 (1992).