Technical Data
CYP21A2, NT (Steroid 21-hydroxylase, Cytochrome P450 21, Cytochrome P450 XXI, 21-OHase, Cytochrome P450-C21, Cytochrome P-450c21, Cytochrome P450-C21B, CYP21, CYP21B)
CYP21A2 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and hydroxylates steroids at the 21 position. Its activity is required for the synthesis of steroid hormones including cortisol and aldosterone. Mutations in CYP21A2 gene cause congenital adrenal hyperplasia.

Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:50-1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
200ul-20CBlue IceHumanRabbit
As reported
Synthetic peptide selected from the N-terminal region of human CYP21A2 (KLH).
Purified by ammonium sulfate precipitation.
Supplied as a liquid in PBS, 0.09% sodium azide.
Recognizes human CYP21A2.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Guerra-Junior,G., Clin. Exp. Immunol. 155(2), 182-188 (2009). 2.Abid,F., Clin. Chem. Lab. Med. 46(12), 1707-1713 (2008). 3.Nelson,D.R., Pharmacogenetics 14(1), 1-18 (2004).