Technical Data
C8995
L-Cysteine, Conjugated
Description:
Applications:
Suitable for use in ELISA and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:1000-1:5000
Immunocytochemistry: 1:1000- 1:5000.
Optimal dilutions to be determined by the researcher.

Cross-Reactivity Results:
Using a conjugate Cysteine-Glutaraldehyde-Protein, antibody specificity was performed with an ELISA test by competition experiments with the following:

Compound ratio: Cross-reactivity results (a):
L-Cysteine-G-(BSA) 1
DL-Homocysteine-G-(BSA) 1:10
L-Methionine-G-(BSA) 1:>1000
L-Glutathion-G-(BSA) 1:>1000 L-Cysteric acid-G-(BSA) 1:>1000
L-Serine-G-(BSA) 1:>1000
Free L-Cysteine 1:>10,000

(a) : Cysteine-G-BSA concentration/unconjugated or conjugated close-related compounds concentration at half displacement

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug4°C (-20°C Glycerol)Blue IceRabbit
Concentration:
~1mg/ml
Immunogen:
Synthetic Cysteine-Glutaraldehyde-BSA.
Purity:
Purified by affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 40% glycerol.
Specificity:
Recognizes protein-conjugated L-Cysteine. See cross-reactivity table for additional data.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.