Technical Data
Cytomegalovirus (CMV)
Human cytomegalovirus is a ubiquitous herpes virus. The primary infection usually occurs in childhood where it most often is asymptomatic. After initial exposure, the virus establishes a life-long infection. In neonates, and in immunocompromised individuals, such as AIDS patients and transplant recipients, HCMV can cause severe life-threatening infections, and it is the most common cause of viral birth defects. Antibody CCH2 reacts with an early nuclear protein identical with the non-structural DNA-binding protein p52. Antibody DDG9 reacts with an immediate early nuclear protein of about 76kD. For both antibodies, the reactivity persists also at later stages during the HCMV infection where the localization is less distinctly nuclear and appears to be in the cytoplasm. Laser confocal microscopy, however, shows that the reaction is limited to the nuclear membrane.

Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry (Paraffin): 1:10-1:25
Immunohistochemistry: Frozen sections
Optimal dilutions to be determined by the researcher.

Positive Control:
Infected lung, human salivary gland

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
MabIgG2a, IgG1,k11E139Supernatant
500ul-20CBlue IceHumanMouse
Not Determined
Cytomegalovirus infected cell lysate. Cellular Localization: Nuclear.
Supplied as a liquid, 0.05% sodium azide.
Recognizes human immediate early and early Cytomegalovirus at 76kD. Does not crossreact with adenovirus, herpes simplex virus, and varicella zoster virus.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Zweygberg et al. J Virol Methods 27: 211, 1990. 2. Leland et al. J Clin Microbiol 27: 1159, 1989. 3. Ashley et al. J Clin Microbiol 27: 2858, 1989. 4. Niedobitek et al. J Clin Pathol 41: 1005, 1988.