Technical Data
Cytokeratins, a group comprising at least 29 different proteins, are characteristic of epithelial and trichocytic cells. Cytokeratin 5, 6 and 8 are members of the type II neutral-to-basic subfamily.Monoclonal antibodies to cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. PCK-26 facilitates the typing of normal, metaplastic and neoplastic cells and it may aid in the discrimination of carcinomas and non-epithelial tumors such as sarcomas, lymphomas and neural tumors. It is also useful in detecting micro-metastases in lymph nodes and other tissues and for determining the origin of poorly differentiated tumors (Lane EB et al,Moll R et al).

Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry (Fr) (paraffin-embedded sections). Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
100ul-20CBlue IceHumanMouse
Not determined
Full length native protein (purified) (Human epidermis).
Ascitic fluid with 0.09% sodium azide
Recognizes the 58kD cytokeratin 5, the 56kD cytokeratin 6 and the 52kD cytokeratin 8 band in immunoblotting (Lane EB et al). It reacts specifically with a wide variety of normal, reactive and neoplastic epithelial tissues. The antibody reacts with simple, cornifying and non-cornifying squamous epithelia and pseudostratified epithelia. It does not react with non-epithelial normal human tissues. Species Crossreactivity: Crossreacts with Chicken, bovine, Goat, Mouse, Rabbit, Rat, Sheep and a wide range of other species.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Lane EB et al. Nature 356:244-6. 2. Lane EB & Alexander CM (1990) Semin Cancer Biol 1:165-79. 3. Moll R et al. (1982) Cell 31:11-24.