Technical Data
C9097-01S
Cytokeratin, Pan
Description:
Cytokeratin filaments consist of a complex family of ~20 different cytokeratin polypeptides with relative molecular weights of 40kD to 68kD. These intracellular intermediate filaments of 10nm in diameter provide structural integrity to epithelial cells. All the filament subunits share a general common fibrillar organization, consisting of a central alpha-helical rod that is flanked by N- and C-terminal domains at each end. Generally, low molecular weight keratins are expressed in the early stages of cell development, whereas high molecular weight keratins are expressed in maturing cells.

Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry (Frozen, paraffin): 1:75-1:200 for paraffin. Incubate 30-60 minutes at RT. Requires Proteolytic enzymatic pretreatment.
Optimal dilutions to be determined by the researcher.

Positive Control:
Skin

Storage and Stability:
May be stored at 4C. For long-term storage, aliquot and store at 4C. Do not freeze. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
500ul4C Do Not FreezeBlue IceBovineRabbit
Concentration:
Not Determined
Immunogen:
Cytokeratin isolated from bovine muzzle epidermis. Cellular Localization: Cytoplasmic.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.5, 15mM sodium azide.
Specificity:
Recognizes bovine cytokeratins of MW 58, 56, 52, 60, 51, 48 and 68kD. Recognizes a broad spectrum of human keratins. Species Crossreactivity: human.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.