Technical Data
C9097-01S
Cytokeratin, Pan
Description:
Cytokeratin filaments consist of a complex family of ~20 different cytokeratin polypeptides with relative molecular weights of 40kD to 68kD. These intracellular intermediate filaments of 10nm in diameter provide structural integrity to epithelial cells. All the filament subunits share a general common fibrillar organization, consisting of a central alpha-helical rod that is flanked by N- and C-terminal domains at each end. Generally, low molecular weight keratins are expressed in the early stages of cell development, whereas high molecular weight keratins are expressed in maturing cells.

Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry (Frozen, paraffin): 1:75-1:200 for paraffin. Incubate 30-60 minutes at RT. Requires Proteolytic enzymatic pretreatment.
Optimal dilutions to be determined by the researcher.

Positive Control:
Skin

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
500ul-20°CBlue IceBovineRabbit
Concentration:
Not Determined
Immunogen:
Cytokeratin isolated from bovine muzzle epidermis. Cellular Localization: Cytoplasmic.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.5, 15mM sodium azide.
Specificity:
Recognizes bovine cytokeratins of MW 58, 56, 52, 60, 51, 48 and 68kD. Recognizes a broad spectrum of human keratins. Species Crossreactivity: human.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.