Technical Data
C9097-01T
Cytokeratin (FITC)
Description:
The C-11 antibody is a broad spectrum antibody which reacts specifically with a wide variety of normal, reactive and neoplastic epithelial tissues. The antibody reacts with simple, cornifying and non-cornifying squamous epithelia and pseudostratified epithelia. It does not react with non-epithelial normal human tissues. This antibody can be applied to methanol- or acetone-fixed frozen sections, and to formalin-fixed, paraffin-embedded human tissues. Increased staining intensity is seen following proteolytic treatment of tested tissue (protease unmasking). Similarly embedded methacarn-fixed material is also suitable for cytokeratin demonstration. This antibody may be used for the localization of cytokeratins using various immunochemical assays including direct immunofluorescence using frozen or fixed tissue sections or cultured cells. It may also be used in flow cytometry of normal and neoplastic epithelial cells. Intermediate-sized filaments are abundant cytoplasmic structural proteins in most vertebrate cells.

Applications:
Suitable for use in Immunofluorescence, Flow Cytometry and Western Blot. Other applications not tested.

Recommended Dilutions:
Immunofluorescence: 1:50
Flow Cytometry: 1:500
Optimal dilutions to be determined by the researcher.

Positive Control:
Ptk2 cells

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.
TypeIsotypeCloneGrade
MabIgG18J1145Affinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Keratin-enriched preparation from cultured human epidermoid carcinoma cell line A431. Cellular Localization: Cyoplasmic
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH7.4, 15mM sodium azide. Labeled with FITC.
Specificity:
Recognizes human cytokeratins 4, 5, 6, 8, 10, 13 and 18 in Western Blot. Species Crossreactivity: Xenopus laevis, bovine, rat, frog
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Bartek, J., et al., (1991) J. Pathol., 164:215. 2. Bartkova, J., et al., (1991) Neoplasma, 38:439. 3. Kasper, M., (1991) Histochemistry, 95:613. 4. Kovarik, J., et al., (1988) Int. J. Cancer, Suppl. 3:50. 5. Lane, E., and Alexander, C., (1990) Sem. Canc. Biol., 1:165. 6. Moll, R., et al, (1982) Cell, 31:11.