Technical Data
C9097-01V
Cytokeratin (PE)
Description:
Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40kD and 68kD. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.

Applications:
Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Flow Cytometry/FACS analysis: 10-20ul per 10e6 cells (100ul cell suspension).
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C before opening. DO NOT FREEZE! Stable for at least 12 months at 4°C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.
TypeIsotypeCloneGrade
MabIgG18J1145Affinity Purified
SizeStorageShippingSourceHost
100ug4°C Do not freezeBlue IceHumanMouse
Concentration:
~0.1mg/ml
Immunogen:
Keratin-enriched preparation from human epidermoid carcinoma cell line A431.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, 15mM sodium azide, 0.2% protease inhibitor. Labeled with R-Phycoerythrin (PE).
Specificity:
Recognizes human Cytokeratin peptides 4, 5, 6, 8, 10, 13, 18. Cytokeratins are a member of intermediate filaments subfamily represented in epithelial tissues. Species Crossreactivity: All (Recognized epitope conserved within all species).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Kovarik J, et al., (1988) Int J Cancer Suppl. 3:50-55. 2. Bartek J, et al., (1991) J Pathol. 164:215-224. 3. Broekema M, et al., (2005) Kidney Int. 68: 2572-2581.