		Technical Data
C9097-04B	Cytokeratin (Cytokeratin 1, CK1, 67kD, Cytokeratin 8, Cytokeratin 19, EHK, EPPK, Hair Alpha Protein, Keratin Type II Cytoskeletal 1, Keratin-1, K1, KRT1, KRT1A, NEPPK, Type-II Keratin Kb1)

Description:
Cytokeratin 1 is a type II Cytokeratin. The type II Cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II Cytokeratin is specifically expressed in the spinous and granular layers of the epidermis with family member KRT10 and mutations in these genes have been associated with bullous congenital ichthyosiform erythroderma.

Applications:
Suitable for use in Western Blot, Immunocytochemistry, Immunofluorescence and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Immunocytochemistry: 1:200
Immunofluorescence: 1:200
Immunohistochemistry: Frozen and paraffin sections.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG2a,k	8J1163	Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice		Mouse

Concentration:

~1mg/ml

Immunogen:

Epidermal keratin

Purity:

Purified

Form

Supplied as a liquid in Tris-glycine, 150mM sodium chloride, pH 7.5, 0.05% sodium azide.

Specificity:

Recognizes cytokeratin 1 at ~67kD. Species Crossreactivity: human.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Machesney M et al. Activated keratinocytes in the epidermis of hypertrophic scars. Am J Pathol 152:1133-41 (1998).