Technical Data
C9097-09B
Cytokeratin 10
Description:
Applications:
Suitable for use in Western blot, Immunocytochemistry and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (Frozen & paraffin sections): 1:5-1:10; Incubate for 1 hour at room temperature. For paraffin sections, protease digestion with trypsin is required.
Optimal dilutions to be determined by the researcher.

Recommended Positive Control:
Squamous cell carcinoma, epidermis.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG15F187Supernatant
SizeStorageShippingSourceHost
1ml4°C (-20°C Glycerol)Blue IceHumanMouse
Concentration:
Not determined
Immunogen:
Cytoskeletal preparation from human epidermis
Purity:
Culture supernatant
Form
Supplied as a liquid in 0.15M PBS, 1% BSA, 0.1% sodium azide.
Specificity:
Recognizes human Cytokeratin 10, Mr 56.6kD, in squamous cells and carcinomas from epidermis, lung, bladder, cervix and esophagus. Species Crossreactivity: most mammals.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Ivanyi, D., et al., J. Pathol. 159: 7-12 (1989).