Description:
Applications:
Suitable for use in Immunoblot and Immunohistochemistry (frozen sections)
Recommended Dilution:
Immunohistochemistry: 1:5-1:10
Positive Control:
Squamous cell carcinoma
Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
|
| Type | Isotype | Clone | Grade |
|---|
| Mab | IgG1 | 5F302 | Supernatant |
|
| Size | | Storage | Shipping | Source | Host |
|---|
| 1ml | | -20°C | Blue Ice | Human | Mouse |
|
| Concentration: |
| Not determined |
| Immunogen: |
| Human skin cell preparation |
| Purity: |
| Culture supernatant |
| Form |
| Supplied as a liquid. |
| Specificity: |
| Can be used for the recognition of keratinizing cells in squamous cell carcinomas from epidermis, lung, bladder, cervix, esophargus etc. The antibody reacts only with suprabasal cells of the epidermis and not with the basal cells. The antibody is directed against the 56.6kD cytokeratin 10 polypeptide. Species Crossreactivity: Human, rat, rabbit, bovine, canine. |
|
| Intended for research use only. Not for use in human, therapeutic, or diagnostic applications. |
Herman C et al. Squamous and Transitional Elements in Rat Bladder Carcinomas Induced by N-butyl-N-4-hydroxybutyl nitrosamine (BBN). Am J Pathol 120: 419-426 (1985).
Smedts F et al. Changing Patterns of Keratin Expression During Progression of Cervical Intraepithelial Neoplasia. Am J Pathol 136: 657-668 (1990).
Langbein L, Pape U-F, Grund C, Kuhn C, Praetzel S, Moll I, Moll R, Franke WW: Tight junction-related structures in the absence of a lumen: Occludin, claudins and tight junction plaque proteins in densly packed cell formations of stratified epithelia and squamous cell carcinomas. Eur J Cell Biol 82, 385-400 (2003)
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