Technical Data
C9097-09C
Cytokeratin 10
Description:
Applications:
Suitable for use in Immunoblot and Immunohistochemistry (frozen sections)

Recommended Dilution:
Immunohistochemistry: 1:5-1:10

Positive Control:
Squamous cell carcinoma

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20C or colder. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG15F302Supernatant
SizeStorageShippingSourceHost
1ml-20CBlue IceHumanMouse
Concentration:
Not determined
Immunogen:
Human skin cell preparation
Purity:
Culture supernatant
Form
Supplied as a liquid.
Specificity:
Can be used for the recognition of keratinizing cells in squamous cell carcinomas from epidermis, lung, bladder, cervix, esophargus etc. The antibody reacts only with suprabasal cells of the epidermis and not with the basal cells. The antibody is directed against the 56.6kD cytokeratin 10 polypeptide. Species Crossreactivity: Human, rat, rabbit, bovine, canine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Herman C et al. Squamous and Transitional Elements in Rat Bladder Carcinomas Induced by N-butyl-N-4-hydroxybutyl nitrosamine (BBN). Am J Pathol 120: 419-426 (1985).
Smedts F et al. Changing Patterns of Keratin Expression During Progression of Cervical Intraepithelial Neoplasia. Am J Pathol 136: 657-668 (1990).
Langbein L, Pape U-F, Grund C, Kuhn C, Praetzel S, Moll I, Moll R, Franke WW: Tight junction-related structures in the absence of a lumen: Occludin, claudins and tight junction plaque proteins in densly packed cell formations of stratified epithelia and squamous cell carcinomas. Eur J Cell Biol 82, 385-400 (2003)