Technical Data
C9097-09G
Cytokeratin 10
Description:
Cytokeratin 10 is a heterotetramer of two type I and two type II keratins. Cytokeratin 10 is generally associated with keratin 1. It is seen in all suprabasal cell layers including stratum corneum. A number of alleles are known that mainly differ in the Gly-rich region (positions 490-560). Defects in cytokeratin 10 are a cause of epidermolytic hyperkeratosis (EHK), also known as bullous congenital ichthyosiform erythroderma (BCIE) or bullous erythroderma ichthyosiformis congenita of Brocq. EHK is an hereditary skin disorder characterized by blistering and a marked thickening of the stratum corneum. At birth, affected individuals usually present with redness, blisters and superficial erosions due to cytolysis. Within a few weeks, the erythroderma and blister formation diminish and hyperkeratoses develop. Transmission is autosomal dominant, but most cases are sporadic. Defects in cytokeratin 10 are also a cause of annular epidermolytic ichthyosis (AEI), also known as cyclic ichthyosis with epidermolytic hyperkeratosis. AEI resembles clinical and histologic features of both epidermolytic hyperkeratosis and ichthyosis bullosa of Siemens.

Applications:
Suitable for use in Immunoprecipitation, Immunohistochemistry and Immunofluorescence. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry (Paraffin): 1:200
Immunohistochemistry (Frozen): 1:10-1:2000
Immunoprecipitation: 1:50
Immunofluorescence (IC): 1:10-1:2000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG18J1167Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Human cytoskeleton preparation extracted from human epidermis by detergent/high salt extraction
Purity:
Purified by affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.
Specificity:
Recognizes and is strongly positive in all suprabasal layers of human epidermis.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.