Technical Data
C9097-11A
Cytokeratin 10
Description:
Cytokeratin 10 (CK10) is a heterotetramer of two type I and two type II keratins. CK10 is typically associated with cytokeratin 1 (CK1). CK10 is expressed in all suprabasal cell layers and it is part of acidic/high molecular weight keratin subfamily. Defects in CK10 causes Epidermolytic hyperkeratosis (EHK), a hereditary skin hereditary skin disorder characterized by blistering and a marked thickening of the stratum corneum. CK10 has also been linked to cause of annular epidermolytic ichthyosis (AEI), also known as cyclic ichthyosis with epidermolytic hyperkeratosis. CK10, along with CK14, 15, 16, and 20, can be used to determine if a neoplasm originated from an epithelial or non-epithelia origin, such as the determination of metastatic malignant tumors of epithelial origin.

Applications:
Suitable for use in Indirect Immunoperoxidase staining, Western Blot and Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:
Indirect Immunoperoxidase: Identifies cytokeratin expressing cells on frozen sections.
Immunohistochemistry: Pretreatment of formalin fixed sections with protease type XXIV (500ug/ml) produces best results.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG12Q1028Highly Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~0.1mg/ml
Immunogen:
Purity:
Purified by ammonium sulfate precipitation followed by ion exchange chromatography.
Form
Supplied as a liquid in PBS, 1% BSA, 0.1% sodium azide.
Specificity:
Recognizes human Cytokeratin 10 found in suprabasal cells of stratified squamous epithelia.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Markley, A., et al., J. Invest. Dermatol. 97: 763-770 (1991). 2. Brockaert, D., et al., Arch. Dematol. Res. 282: 383391 (1990).