Technical Data
C9097-11C
Cytokeratin 10
Description:
Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40kD and 68kD and isoelectric pH between 4.9–7.8. The individual cytokeratin polypeptides are designated 1 to 20. Cytokeratin 1 has the highest molecular weight and the highest isoelectric pH, while cytokeratin 19 has the lowest molecular weight and a low isoelectric pH. The cytokeratins are divided into the type I and type II subgroups, the type II family members comprising the basic to neutral cytokeratins 1-8, while the type I group comprises the acidic cytokeratins 9-20. The cytokeratins are expressed in combinations which characterize the type of epithelium.

Applications:
Suitable for use in Western Blot, Immunocytochemistry, Immunohistochemistry and Flow Cytometry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:50-1:500
Immunohistochemistry: Frozen tissue sections. Avidin-biotinylated horseradish peroxidase complex (ABC) as a detection reagent.
Flow Cytometry: 1:50-1:100
Optimal dilutions to be determined by researcher.

Hybridoma:
Hybridization of SP2/0 myeloma cells with spleen cells from mice immunized with cytokeratins from human epidermis.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG14A26Affinity Purified
SizeStorageShippingSourceHost
100ug4°C (-20°C Glycerol)Blue IceHumanMouse
Concentration:
~0.5mg/ml
Immunogen:
Cytokeratins from human epidermis.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide before the addition of glycerol to 40%. No stabilizing proteins added.
Specificity:
Reacts with keratinizing stratified epithelia and in differentiated areas of highly differentiated squamous cell carcinomas. Reacts exclusively with human Cytokeratin 10. Species Crossreactivity: mouse, rat and canine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Ramaekers F., et al., Histochem. J. 15: 691-713 (1983). 2. Puts, J.J., et al., Int. J. Gynecol. Pathol. 4: 300-313 (1985). 3. Broers, J.L., et al., J. Cell Sci. 83: 37-60 (1986). 4. Ramaekers, F., et al., Exp. Cell Res. 170: 235-249 (1987). 5. van Muijen, G.N., et al., Exp. Cell Res. 171: 331-345 (1987). 6. Ramaekers, F., et al., Am. J. Pathol. 136: 641-655 (1990). 7. Schaafsma, H.E., et al., Am. J. Pathol. 136: 329-343 (1990). 8. Smedts, F., et al., Am. J. Pathol. 136: 657-658 (1990).