Technical Data
C9097-11E
Cytokeratin 10,13
Description:
Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40kD and 68kD and isoelectric pH between 4.9 – 7.8. The individual cytokeratin polypeptides are designated 1 to 20. Cytokeratin 1 has the highest molecular weight and the highest isoelectric pH, while cytokeratin 19 has the lowest molecular weight and a low isoelectric pH. The cytokeratins are divided into the type I and type II subgroups, the type II family members comprising the basic to neutral cytokeratins 1-8, while the type I group comprises the acidic cytokeratins 9-20. The cytokeratins are expressed in combinations which characterize the type of epithelium.

Applications:
Suitable for use in Immunohistochemistry (frozen and paraffin sections), Flow Cytometry, Immunocytochemistry, and Western Blotting. Other applications not tested.

Optimum dilutions to be determined by researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2a4A28Affinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanMouse
Concentration:
1mg/ml
Immunogen:
Cytokeratin 10,13
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide. No stabilizing proteins added.
Specificity:
Reacts with non-keratinizing stratified epithelia and transitional epithelium, as well as carcinomas derived from these tissues. This antibody reacts particularly well in paraffin embedded tissue where it only recognizes cytokeratin 13. Species crossreactivity: Human, feline.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Smedts F, et al., Am. J. Path. 136: 657-668 (1990). 2. Ramaekers F, et al. Exp. Cell Res. 170: 235-249 (1987). 3. Smedts F, et al. Am. J. Path. 141: 497-511 (1992). 4. Raats JMH, et al. J. Cell Biol. 111: 1971-1985 (1990). 5. Ramaekers FCS, et al. Am. J. Pathol. 136: 641-655 (1990). 6. Puts JJG, et al. Int. J. Gynecol. Pathol. 4: 300-313 (1985). 7. Wetzels RHW,et al. Am. J. Path. 138: 751-763 (1991). 8. Wetzels RHW, et al. Histopathol. 20: 295-303 (1992).  9. Ramaekers F, et al. Lab. Invest. 49: 353-361 (1983). 10. Schaafsma HE,et al.Am. J. Path. 136: 329-343 (1990).