Description:
Applications:
Suitable for use in Immunofluorescence, Immunohistochemistry (frozen and paraffin-embedded tissue and cytological material; protease pretreatment improves the performance of this excellent marker) and Immunoblot.
Recommended Dilution:
Immunohistochemistry: 1:10 with PBS, pH 7.4; Incubation Time 1 hr at RT; extended with paraffin.
Storage and Stability:
Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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| Type | Isotype | Clone | Grade |
|---|
| Mab | IgG1 | 5F225 | Affinity Purified |
|
| Concentration: |
| ~0.05mg/ml |
| Immunogen: |
| Cytokeratin 13 of Mr 54,000 purified from human esophagus. |
| Purity: |
| Purified by Protein A affinity chromatography. |
| Form |
| Supplied as a lyophilized powder in PBS, pH 7.4, 0.5% BSA, 0.09% sodium azide. Reconstitute to a final volume of 1ml. |
| Specificity: |
| Antigen Recognized in Human, bovine and rat. Reactivities on Cultured Cell lines from squamous cell CA. Cell Lines (tested so far) e.g. A-431 from epidermoid CA of vulva; RT 112, RT-4 of urinary bladder. Represents an excellent marker to discriminate non-cornified squamous epithelia from those of different origin. Polypeptide reacting: Mr 54 000 polypeptide (human cytokeratin 13 with minor affinity to cytokeratins 14, Mr 50,000, and 16, Mr 48,000. Tumors specifically detected: several squamous cell carcinomas, e.g. cervix carcinoma; transitional cell carcinoma of the bladder; craniopharyngioma. |
|
| Intended for research use only. Not for use in human, therapeutic, or diagnostic applications. |
Dallenbach-Hellweg, G. and Lang, G.: Immunohistochemical studies on uterine tumors. Path. Res. Pract. 187, 36-43 (1991).
Dockhorn-Dworniczak, B., Franke, W.W., Schröder, S., Czernobilsky, B., Gould, V.E. and Böcker, W.: Patterns of expression of cytoskeletal proteins in human thyroid gland and thyroid carcinomas. Differentiation 35, 53-71 (1987).
Franke, W.W., Moll, R., Achtstätter Th. and Kuhn, C.: Cell typing of epithelia and carcinomas of the female genital tract using cytoskeletal proteins as markers. Banbury Report 21: Viral Etiology of Cervical Cancer, Cold Spring Harbor Laboratory (NY), 121-148 (1986).
Moll, R., Achtstätter, Th., Becht, E., Balcarova-Ständer, J., Ittensohn, M. and Franke, W.W.: Cytokeratins in normal and malignant transitional epithelium. Am. J. Pathol. 132, 123-144 (1988).
Moll, R., Franke, W.W., Schiller, D.L., Geiger, B. and Krepler, R.: The catalog of human cytokeratins: Patterns of expression in normal epithelia, tumors and cultured cells. Cell 31, 11-24 (1982).
Demirkesen C, Hoede N, Moll R: Epithelial markers and differentiation in adnexal neoplasms of the skin: an immunohistochemical study including individual cytokeratins. J Cutan Pathol 22: 518-535 (1995).
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