Description:
Applications:
Suitable for Immunofluorescence, Immunoblot and Immunohistochemistry (frozen sections).
Recommended Dilution:
Immunohistochemistry: 1:5-1:20
Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
|
| Type | Isotype | Clone | Grade |
|---|
| Mab | IgG2b | 5F96 | Supernatant |
|
| Size | | Storage | Shipping | Source | Host |
|---|
| 1ml | | -20°C | Blue Ice | Human | Mouse |
|
| Concentration: |
| Not determined |
| Immunogen: |
| Cytokeratin preparation from human esophagus. |
| Purity: |
| Culture supernatant |
| Form |
| Supplied as a liquid. |
| Specificity: |
| Antigen Recognized in Species (tested so far) Human, most mammals incl. mouse, rat, rabbit. Specifically recognizes cytokeratin 13 (54kD polypeptide) present in non-cornifying squamous epithelia and in transitional epithelium of the bladder. Epidermal cell culture studies have shown that cytokeratin 13 expression is involved in either the number or the strength of desmosomal contacts between suprabasal cells of stratified squamous epithelia. |
|
| Intended for research use only. Not for use in human, therapeutic, or diagnostic applications. |
Muijen, GNP van et al. Cell type heterogeneity of cytokeratin expression in complex epithelia and carcinomas as demonstrated by monoclonal antibodies specific for cytokeratins nos. 4 and 13. Exp Cell Res 162:97-113
Kopan, R et al. Retinoids as important regulators of terminal differentiation. Examining keratin expression in individual epidermal cells at various stages of keratinization. J Cell Biol 105:427-440
Smedts, F et al. Changing Patterns of Keratin Expression During Progression of Cervical Intraepithelial Neoplasia. Am J Pathol 136:657-668
|
|
