Cytokeratin 13 (54kD polypeptide) present in non-cornifying squamous epithelia and in transitional epithelium of the bladder. Epidermal cell culture studies have shown that cytokeratin 13 expression is involved in either the number or the strength of desmosomal contacts between suprabasal cells of stratified squamous epithelia.
Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry. Other applications not tested.
Western Blot: 1:5-1:10
Immunohistochemistry (Frozen): 1:5-1:10
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
| Not determined|
|Cytokeratin preparation from human esophagus.|
|Supplied as a liquid in 0.09% sodium azide.|
|Recognizes human Cytokeratin 13, 54kD. Species Crossreactivity: mouse, rat, rabbit and most mammals|
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
Muijen, GNP van et al. Cell type heterogeneity of cytokeratin expression in complex epithelia and carcinomas as demonstrated by monoclonal antibodies specific for cytokeratins nos. 4 and 13. Exp Cell Res 162:97-113|
Kopan, R et al. Retinoids as important regulators of terminal differentiation. Examining keratin expression in individual epidermal cells at various stages of keratinization. J Cell Biol 105:427-440
Smedts, F et al. Changing Patterns of Keratin Expression During Progression of Cervical Intraepithelial Neoplasia. Am J Pathol 136:657-668