Technical Data
C9097-13A
Cytokeratin 14
Description:
Cytokeratin 14 is one of two cytokeratins that can distinguish stratifying epithelial cell types from simple epithelial cell types, being expressed by stratifying/keratinocytes cell types but not by simple epithelial cells.

Applications:
Suitable for use in Flow Cytometry, Immunohistochemistry, Western Blot and Immunofluorescence. Other applications not tested.

Recommended Dilutions:
Flow Cytometry: 1:100. Use10ul to label 10e6 cells in 100ul. Membrane permeabilization is required.
Immunohistochemistry: frozen sections
Immunohistochemistry (paraffin): 1:200. Requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer is recommended for this purpose.
Optimal dilutions to be determined by the researcher.

Recommended Secondary Antibodies:
I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
I1904-14C: IgG, Fc (HRP) Pab Gt xMo
I1904-19C: IgG, F(ab’)2 (HRP) Pab Gt xMo

Recommended Positive Control Tissue:
Skin

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG33H2053Highly Purified
SizeStorageShippingSourceHost
200ug-20°CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Last 15 C-terminal residues of human cytokeratin 14 conjugated to thyroglobulin.
Purity:
Purified by Ion Exchange chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity:
Recognizes human cytokeratin 14, a type I intermediate filament. Species Crossreactivity: elephant, canine, porcine
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Purkis, P.E. et al. (1990) J. Cell Sci. 97: 39-50. 2. Lane, E.B. & Alexander, C.M. (1990). Semin. Canc. Biol. 1: 165-179. 3. Westzels, R.H.W. et al. (1989) Am. J. Pathol. 134: 571-579. 4. Moll, R. et al. (1982) Cell. 31: 11-24. 5. Richardson, G. et al. (2004) J. Cell Sci. 117:3539-3545. 6. Holliday, D. et al. (2009) Breast Cancer Res. 11: R3. 7. Eastman, R. .Jr. et al. (2010) Am J Physiol Renal Physiol.299:F1094-110. 8. Stumpf, P and Welsch, U. (2004) (Loxodonta africana, Proboscidea) Zoomorphology 123:155–167