Description:
In several studies the correlation between the expression of keratin 14 in in different types of carcinomas (putatively derived from basal epithelial cells) and prognosis has been discussed.
Applications:
Suitable for use in Immunofluorescence microscopy, Immunoblot and Immunohistochemistry (frozen sections).
Recommended Dilution:
Immunohistochemistry: 1:5-1:10
Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
|
| Type | Isotype | Clone | Grade |
|---|
| Mab | IgG1 | 5F298 | Supernatant |
|
| Size | | Storage | Shipping | Source | Host |
|---|
| 1ml | | -20°C | Blue Ice | Human | Mouse |
|
| Concentration: |
| Not determined |
| Immunogen: |
| Isolated from TR146 cells (human tumor cell line) |
| Purity: |
| Culture supernatant |
| Form |
| Supplied as a liquid. |
| Specificity: |
| Reacts with cytokeratin 14, expressed in the basal cells of the larynx, esophagus, trachea, bladder, prostate, cervix, vagina, breast acini, skin and sweat glands. Species Crossreactivity: Human, porcine, canine. |
|
| Intended for research use only. Not for use in human, therapeutic, or diagnostic applications. |
Wetzels RHW et al. Detection of basement membrane component and basal cell keratin 14 in noninvasive carcinomas of the breast. Am J Pathol 134:571-579 (1989)
Smedts F et al. Keratin Expression in Cervical Cancer. Am J Pathol 141:497-511 (1992)
Wetzels RHW et al. Laminin and Type VII Collagen Distribution in Different Types of Human Lung Carcinoma: Correlation with Expression of Keratins 14, 16, 17, and 18. Histopathology 20:295-303
Demirkesen C, Hoede N, Moll R: Epithelial markers and differentiation in adnexal neoplasms of the skin: an immunohistochemical study including individual cytokeratins. J Cutan Pathol 22: 518-535 (1995).
Moll R. et al.: Differenzierungsmarker bei gynäkologischen Tumoren: Methodische und diagnostische Aspekte. In: Aktuelle Aspekte der Tumorimmunologie in der Gynäkologie. W. Zuckschwerdt Verlag (1995).
|
|
