Technical Data
C9097-13G
Cytokeratin 14 (KRT14, Keratin 14, CK14, EBS3, EBS4, K14, Keratin, type I cytoskeletal 14, NFJ)
Description:
Cytokeratin 14 is a type I intermediate filament. It is one of the two cytokeratins that distinguish stratifying epithelial cells types from simple epithelial cell types. Cytokeratin 14 is expressed by stratifying/keratinocyte cell types, but not by simple epithelial cells.

Applications:
Suitable for use in Western Blot, Immunocytochemistry and Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:
Western Blot: 1:5000-1:10,000
Immunohistochemistry (Frozen): 1:100-1:200; Incubation Time: 1 hr at RT.
Immunohistochemistry (Paraffin-embedded tissue): 1:50; Incubation Time: >1 hr at RT
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanGuinea pig
Concentration:
Not Determined
Immunogen:
Recombinant protein corresponding to human cytokeratin 14 (complete polypeptide).
Purity:
Serum
Form
Supplied as a liquid in 0.5% BSA, 0.09% sodium azide.
Specificity:
Recognizes acidic human cytokeratin 14 expressed in the basal cells of the larynx, esophagus, trachea, bladder, prostate, cervix, vagina, breast acini, skin and sweat glands. Species Crossreactivity: bovine and mouse
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Demirkesen, C., et al., (1995) J. Cutan. Pathol. 22: 518-535. 2. Moll, R., et al., (1995) W. Zuckschwerdt Verlag. 3. Hofmann, I., et al.,(2000) J. Cell Science 113: 2471-2483. 4. Herrmann, T., et al., (2003) J. Cell Biol. 161: 1105-1115. 5. Langbein, L., et al., (2003) Eur. J. Cell Biol. 28: 385-400. 6. Jennemann R, et al., (2007) J. Biol Chem 282: 3083-3094.