Technical Data
C9097-13T
Cytokeratin 14 (FITC)
Description:
Cytokeratin 14, a type I intermediate filament, is one of the two cytokeratins that distinguish stratifying epithelial cells types from simple epithelial cell types.Thus, cytokeratin 14 is expressed by stratifying/keratinocyte cell types but not by simple epithelial cells.

Applications:
Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Flow Cytometry: Use 10ul to label 10e6 cells.
Immunohistochemistry (Frozen/Paraffin): For paraffin sections, microwave treatment or high temperature antigen unmasking technique is recommended. Prior exposure of fixed tissues to enzyme (trypsin) digestion is deleterious to epitope expression. The fixative recommended is neutral buffered formalin.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.
TypeIsotypeCloneGrade
MabIgG38J1174Purified
SizeStorageShippingSourceHost
50 Tests-20°CBlue IceHumanMouse
Concentration:
Not Determined
Immunogen:
Synthetic peptide corresponding to the extreme carboxy terminal of human keratin 14 (last 15 residues). (Thyroglobulin). Cellular Localization: Cytoplasmic.
Purity:
Purified
Form
Supplied as a liquid in PBS, 1% BSA.,10mM sodium azide. Labeled with Fluorescein isothiocyanate (FITC).
Specificity:
Recognizes human cytokeratin 14.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Purkis, et al. (1990) J Cell Sci. 97:39-50.