Technical Data
C9097-14B
Cytokeratin 14
Description:
Cytokeratin 14 is a type I intermediate filament. It is one of the two cytokeratins that distinguish stratifying epithelial cells types from simple epithelial cell types. Cytokeratin 14 is expressed by stratifying/keratinocyte cell types but not by simple epithelial cells.

Applications:
Suitable for use in Immunohistochemistry and Western Blot. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry: Suitable for staining formalin fixed wax sections or frozen tissues. Recommended to either microwave treat fixed tissue sections or high temperature antigen unmasking technique. Prior exposure of fixed tissues to enzyme (trypsin) digestion is deleterious to epitope expression. The fixative recommended for use is neutral buffered formal saline
Optimal dilutions to be determined by researcher.

Hybridoma:
NS1 myeloma cells with spleen cells from Balb/c mice.

Source: Tissue culture supernatant

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add glycerol (40-50%) and aliquot. Store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG32Q1030Highly Purified
SizeStorageShippingSourceHost
100ug4C Do Not FreezeBlue IceHumanMouse
Concentration:
~0.1mg/ml
Immunogen:
Synthetic peptide corresponding to the last 15 residues of extreme C-terminus of human keratin 14 (Thyroglobulin coupled).
Purity:
Purity by ammonium sulfate precipitation followed by ion exchange chromatography.
Form
Supplied as liquid in PBS, 10mM sodium azide, 1mg/ml BSA.
Specificity:
Recognizes human Cytokeratin 14.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
US Biological application references: 1. Zhang, C. et al., (2011) PLoS ONE 6(8): e23849. doi:10.1371/journal.pone.0023849 2. Dakir, E. H. et al., (2008) Carcinogenesis 28:2377-2384. 1. Moll, R., et al., Cell 31: 11-14 (1982). 2. Purkis, P.E., et al., J. Cell Sci. 97: 39-50 (1990).