Technical Data
C9097-14D
Cytokeratin 14 (KRT14, Keratin 14, CK14, EBS3, EBS4, K14, Keratin, type I cytoskeletal 14, NFJ) (FITC)
Description:
Cytokeratin 14 is a type I intermediate filament. It is one of the two cytokeratins that distinguish stratifying epithelial cells types from simple epithelial cell types. Cytokeratin 14 is expressed by stratifying/keratinocyte cell types but not by simple epithelial cells.

Applications:
Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Flow Cytometry: 10ul per test.
Immunohistochemistry (Formalin fixed paraffin sections or frozen tissues): Recommended to either microwave treat fixed tissue sections or use high temperature antigen unmasking technique. Prior exposure of fixed tissues to enzyme (trypsin) digestion is deleterious to epitope expression. The fixative recommended for use is neutral buffered formalin.
Optimal dilutions to be determined by researcher.

Hybridoma:
NS1 myeloma cells with spleen cells from Balb/c mice.

Source:
Tissue culture supernatant

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG32Q1030Highly Purified
SizeStorageShippingSourceHost
100 Tests-20°CBlue IceHumanMouse
Concentration:
~100tests/ml
Immunogen:
Synthetic peptide corresponding to the last 15 residues of extreme C-terminus of human keratin 14 (Thyroglobulin coupled).
Purity:
Purity by ammonium sulfate precipitation followed by ion exchange chromatography.
Form
Supplied as liquid in PBS, 10mM sodium azide, 1mg/ml BSA. Labeled with fluorescein isothiocyanate (FITC).
Specificity:
Recognizes human Cytokeratin 14.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Moll, R., et al., Cell 31: 11-14 (1982). 2. Purkis, P.E., et al., J. Cell Sci. 97: 39-50 (1990). 3. Wang, D., et al., Invest. Opthamol. Vis. Sci. 44: 4698-4704 (2003).