Technical Data
C9097-15P4
KRT16 (Keratin, Type I Cytoskeletal 16, Cytokeratin-16, CK-16, Keratin-16, K16, KRT16A)
Description:
Among the cytoplasmic intermediate filaments (IF) proteins, keratins make up the largest family and are expressed specifically in epithelial cells in a cell-specific manner. Keratins include more than 20 unique gene products (termed K1-K20) that are divided into type I (K9-K20) and type II (K1-K8). Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains and are clustered in a region of chromosome 17q12-q21. Keratin 16 is a type-I keratin which associates with KRT6 isomers. Mutations in this gene are associated with type 1 pachyonychia congenita, non-epidermolytic palmoplantar keratoderma and unilateral palmoplantar verrucous nevus. It maps to 17q12-q21 region of human chromosome. It is expressed in the hair follicle, nail bed and in mucosal stratified squamous epithelia and, suprabasally, in oral epithelium and palmoplantar epidermis and also found in luminal cells of sweat and mammary gland ducts. Defects in KRT16 are a cause of pachyonychia congenita type 1, nonepidermolytic palmoplantar keratoderma and unilateral palmoplantar verrucous nevus.

Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (Formalin fixed paraffin embedded): 1:25-1:100
Immunohistochemistry (Frozen): 1:100-1:200
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabPurified
SizeStorageShippingSourceHost
125ul-20CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Synthetic peptide corresponding to human Cytokeratin 16 conjugated to KLH.
Purity:
Purified
Form
Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 20mg/ml BSA, 0.05% sodium azide.
Specificity:
Recognizes human KRT16.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.