Technical Data
C9097-23D3
Cytokeratin 18 (FITC)
Description:
C9097-23D3 represents an excellent marker to discriminate simple epithelia from those of different origin. Tumors specifically detected: all adenocarcinoma; mammary carcinoma, urinary bladder carcinoma, undifferentiated carcinoma, cervix carcinoma, hepatocellular carcinoma.

Applications:
Suitable for use in Flow Cytometry, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (Frozen, Paraffin-embedded): 1:10; 1hr at 37C.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG15F227Affinity Purified
SizeStorageShippingSourceHost
250ul-20CBlue IceHumanMouse
Concentration:
Not Determined
Immunogen:
Human cytokeratin 18 from HeLa cytoskeletal preparation.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid, 0.09% sodium azide. Labled with fluorescein isothiocyanate (FITC).
Specificity:
Recognizes Cytokeratin 18 (MW: 45kD) of all human simple type epithelia and basal cells of many squamous, nonepidermal epithelia. Reacts with cultured HeLa and MCF-7 cells. Species Crossreactivity: mouse, rat, bovine, porcine, canine, hamster, sheep, fish (trout).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Bartek, J., et al., J. Pathol. 164: 215-224 (1991). 2. Lanerova, L., et al., Hybridoma 7: 495-504 (1988). 3. Markl, J., et al., Eur. J. Cell Biol. 50: 1-16 (1989). 4. Moll, R., et al., Cell 31: 11-24 (1982). 5. Gomi, H., et al., Neuron 14: 29-41 (1995). 6. Bantel, H., et al., Eur. J. Cell Biol. 80: 230-239 (2001). 7. Stumptner, C., et al., Hepatology 35: 1053-1062 (2002). 8. Ikenouchi, J., et al., J. Cell. Sci. 116: 1959-1967 (2003).