Technical Data
C9097-34M
Cytokeratin 3, K2P (Keratin K3/K76)
Description:
Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40kD and 68kD and isoelectric pH between 4.9-7.8. The individual cytokeratin polypeptides are designated 1 to 20. The cytokeratins are expressed in combinations which characterize the type of epithelium.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:
Western Blot: 1:500
Immunohistochemistry (Frozen): 1:50
Immunohistochemistry (Paraffin): 1:50 on paraffin-embedded tissue sections fixed in 10% neutral buffered formalin or Fekete’s acid-alcohol-formalin following trypsin digestion antigen retrieval.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG12Q1040Highly Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceRabbitMouse
Concentration:
~1mg/ml
Immunogen:
Rabbit corneal epithelial keratin
Purity:
Purified by ion exchange chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity:
Recognizes the 64kD polypeptide (cytokeratin 3) of rabbit corneal epithelium and keratin K76 (cytokeratin K2p) of palate epithelium. As such represents an excellent marker for studying corneal epithelial-type differentiation. Also recognizes bovine snout and lip epithelium, as well as a rabbit corneal cell line. Species Crossreactivity: human, bovine and mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Mikaelian, I., et al., Toxicology Pathology 32: 181-191 (2004). 2. Wang, D., et al., Invest. Ophthalmol. Vis. Sci. 44: 4698-4704 (2003). 3. Schermer, A., et al., J. Cell Biol. 103: 49-62 (1986). 4. Cooper, D., et al., Lab. Invest. 52: 243-256 (1985). 5. Moll, R., et al., Cell 31: 11-24 (1982). 6. Sun, T.T. and Green, H., Cell 14: 469-476 (1978).