Technical Data
C9097-37
Cytokeratin 5 (CK 5, CK5, CK-5, 58kD Cytokeratin, DDD, EBS 2, EBS2, Keratin 5 (Epidermolysis Bullosa Simplex Dowling Meara/Kobner/Weber Cockayne Types), Keratin-5, Keratin Type II Cytoskeletal 5, K5, KRT5, KRT5A)
Description:
Cytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinizing tissue (ie., skin) and non keratinizing cells (ie., mesothelial cells). Although not a traditional marker for endothelial cells, cytokeratins have also been found in some microvascular endothelial cells. At least 20 different cytokeratins (CK) in the molecular range of 40 to 70kD and isoelectric points of 5 to 8.5 can be identified using two dimensional gel electrophoresis. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells. Defects in KRT5 are a cause of epidermolysis bullosa simplex.

Applications:
Suitable for use in Immunohistochemistry and Western Blot. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry (Frozen): 1:100, Incubation time 1hr at RT.
Immunohistochemistry (Paraffin): 1:50, Incubation time >1hr at RT.
Western Blot (ECL): 1:5000-1:10,000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
100ul-20°CBlue IceHumanGuinea pig
Concentration:
Not Determined
Immunogen:
Recombinant human cytokeratin 5
Purity:
Serum
Form
Supplied as a liquid in 0.5% BSA, 0.09% sodium azide.
Specificity:
Recognizes human basic cytokeratin 5 (Mr 58,000), expressed in the basal and suprabasal layers. Recognizes basal cells of the larynx, esophagus, trachea, bladder, prostate, cervix, vagina, breast acini, skin and sweat glands. Species Crossreactivity: bovine and mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Demirkesen C, Hoede N, Moll R: Epithelial markers and differentiation in adnexal neoplasms of the skin: an immunohistochemical study including individual cytokeratins. J Cutan Pathol 22: 518-535 (1995). Moll R. et al.: Differenzierungsmarker bei gynäkologischen Tumoren: Methodische und diagnostische Aspekte. In: Aktuelle Aspekte der Tumorimmunologie in der Gynäkologie. W. Zuckschwerdt Verlag (1995).